pGLO Lab Report

pGLO Lab














Our experiments within the test tubes
LB/AMP and LB/AMP/ARA Petri dishes, pGLO+

Our colonies of E.Coli

Our dishes with the UV Light

Our dishes without the UV light

Conclusions / Results:
This experiment turned out well because we were able to produce hundreds of E.Coli colonies in our pGLO+ LB/AMP/ARA dish. We learned about the production of DNA and how we can combine this bacteria, in this case E.Coli, with the GFP or green fluorescent protein. By doing this, we are able to see how quickly the E.Coli reproduces under certain circumstances. We used ampicillin (AMP) to kill the bacteria in the dishes without Arabinose (ARA). However, our dish with lysogeny broth (LB) in presence with arabinose, creates beta lactamase which is a resist protein to ampicillin. This also results in the expression of the GFP protein, giving the E.Coli its fluorescent glow in the UV light.

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